Application of Web Services to a Simulation Framework

The Joint Semi-Automated Forces (JSAF) simulator is an excellent tool for military training and a great testbed for new SAF behaviors. However, it has the drawback that behaviors must be ported into its own Finite State Machine (FSM) language. Web Services is a growing technology that seamlessly connects service providers to service consumers. This work attempts to merge these two technologies by modeling SAF behaviors as web services. The JSAF simulator is then modeled as a web service consumer. This approach allows new Semi-Automated Forces (SAF) behaviors to be developed independently of the simulator, which provides the developer with greater flexibility when choosing a programming language, development environment, and development platform. In addition to new SAF behaviors, this approach also supports any external component that can be modeled as a web service. Furthermore, these services are often run over a network, which distributes the computational load across several computers. Finally, hosting copies of a single service on several machines, a concept similar to file-sharing mirrors, offers an environment for load-balancing. This means if several entities are running the same behavior, a single server does not perform the computation for every entity. Instead, each entity is assigned to a specific server, increasing the quality of service seen by the system. A Web Services framework linking JSAF with several services is designed and implemented. Suppression of Enemy Air Defense (SEAD) behaviors written in MATLAB and a behavior recognition system are integrated with JSAF. These behaviors and the recognition tool were developed by other researchers, independent of this work. Results show that offloading computation to other machines is beneficial, especially when the simulation system is under heavy load. Preliminary results also indicate that load-balancing performs much better than using a single server

Normalized ghost imaging

We present an experimental comparison between different iterative ghost imaging algorithms. Our experimental setup utilizes a spatial light modulator for generating known random light fields to illuminate a partially-transmissive object. We adapt the weighting factor used in the traditional ghost imaging algorithm to account for changes in the efficiency of the generated light field. We show that our normalized weighting algorithm can match the performance of differential ghost imaging

Fast full-color computational imaging with single-pixel detectors

Single-pixel detectors can be used as imaging devices by making use of structured illumination. These systems work by correlating a changing incident light field with signals measured on a photodiode to derive an image of an object. In this work we demonstrate a system that utilizes a digital light projector to illuminate a scene with approximately 1300 different light patterns every second and correlate these with the back scattered light measured by three spectrally-filtered single-pixel photodetectors to produce a full-color high-quality image in a few seconds of data acquisition. We utilize a differential light projection method to self normalize the measured signals, improving the reconstruction quality whilst making the system robust to external sources of noise. This technique can readily be extended for imaging applications at non-visible wavebands

Heralding efficiency and correlated-mode coupling of near-IR fiber-coupled photon pairs

We report on a systematic experimental study of the heralding efficiency and generation rate of telecom-band infrared photon pairs generated by spontaneous parametric down-conversion and coupled to single-mode optical fibers. We define the correlated-mode coupling efficiency, an inherent source efficiency, and explain its relation to heralding efficiency. For our experiment, we developed a reconfigurable computer-controlled pump-beam and collection-mode optical apparatus which we used to measure the generation rate and correlated-mode coupling efficiency. The use of low-noise, high-efficiency superconducting nanowire single-photon detectors in this setup allowed us to explore focus configurations with low overall photon flux. The measured data agree well with theory, and we demonstrated a correlated-mode coupling efficiency of 97% ± 2%, which is the highest efficiency yet achieved for this type of system. These results confirm theoretical treatments and demonstrate that very high overall heralding efficiencies can, in principle, be achieved in quantum optical systems. It is expected that these results and techniques will be widely incorporated into future systems that require, or benefit from, a high heralding efficiency.United States. Dept. of Defense. Assistant Secretary of Defense for Research & Engineering (Air Force Contract FA8721-05-C-0002

Dietary cooked navy beans and their fractions attenuate colon carcinogenesis in azoxymethane-induced ob/ob mice

Based on the protective effects of cooked dry bean consumption in a human intervention study, we evaluated which fraction of cooked dry beans is responsible for its cancer-preventive effects. Cooked navy beans (whole beans), the insoluble fraction (bean residue) or soluble fraction of the 60% (vol:vol) ethanol extract of cooked navy beans (bean extract), or a modified AIN-93G diet (16.6% fat including 12.9% lard) as control diet were fed to 160 male obese ob/ob mice after 2 azoxymethane injections. In comparison to control-fed mice, dysplasia, adenomas, or adenocarcinomas were detected in fewer mice on either bean fraction diet (percent reduction from control: whole beans 54%, P = 0.10; bean residue 81%, P = 0.003 ; bean extract 91%, P = 0.007) , and any type of colon lesions, including focal hyperplasia, were found in fewer mice on each of the 3 bean diets percent reduction from control: whole bean 56%, P= 0.04; bean residue 67%, P = 0.01; bean extract 87%, 373 374 G. BOBE ET AL. P = 0.0003. These results suggest that both the soluble and the insoluble fraction of the extract contribute to the cancer-protective effect of cooked navy beans. INTRODUCTION Colorectal cancer (CRC) is the third most commonly diagnosed type of cancer and the fourth most common cause of cancer-related death worldwide (1). In the United States, CRC is the fourth leading cancer in incidence rates and the second leading cause of cancer-related mortality with a 5-yr survival rate of 64% (2). Despite the effectiveness of screening (3,4), there is limited impact for CRC prevention because of low screening rates (5). Nutrition remains critical for CRC prevention. It is estimated that nutrition could prevent 70-80% of all CRC cases (6). This is important, as the annual CRC treatment costs in the United States are estimated to be $6.5 billion (7). Two of the main risk factors for CRC, which are both diet related, are obesity and inflammation (8). Thus, ob/ob mice might provide a suitable animal model to study the link between diet and CRC because they have a mutation in the leptin gene, which results in hyperphagia, obesity, hyperinsulinemia, hyperglycemia, and increased inflammatory response to liposaccharides (9). Dry beans (Phaseolus vulgaris L.), which belong to the Leguminosae family, are a dietary staple in many Latin American, Eastern, and South African countries that potentially could prevent CRC (10,11). Ecological analysis indicates a decreased risk of death associated with colon cancer in countries with higher consumption of beans (12). In studies that have examined the association between colon cancer and individual intakes of legumes, the results indicate a protective effect in populations with higher legume consumption (13-18). In the only study that examined the effect of dry bean consumption separately, male participants, who consumed at least 31 g of cooked dry beans daily, had reduced risk of advanced adenomatous polyp recurrence in a 4-yr nutrition intervention study (Polyp Prevention Trial (19); unpublished data). In animal models, dry beans commonly consumed in the United States, such as pinto, black, and navy beans, reduced azoxymethane(AOM)-induced colon adenocarcinomas in F344 rat

A conscious mouse model of gastric ileus using clinically relevant endpoints

BACKGROUND: Gastric ileus is an unsolved clinical problem and current treatment is limited to supportive measures. Models of ileus using anesthetized animals, muscle strips or isolated smooth muscle cells do not adequately reproduce the clinical situation. Thus, previous studies using these techniques have not led to a clear understanding of the pathophysiology of ileus. The feasibility of using food intake and fecal output as simple, clinically relevant endpoints for monitoring ileus in a conscious mouse model was evaluated by assessing the severity and time course of various insults known to cause ileus. METHODS: Delayed food intake and fecal output associated with ileus was monitored after intraperitoneal injection of endotoxin, laparotomy with bowel manipulation, thermal injury or cerulein induced acute pancreatitis. The correlation of decreased food intake after endotoxin injection with gastric ileus was validated by measuring gastric emptying. The effect of endotoxin on general activity level and feeding behavior was also determined. Small bowel transit was measured using a phenol red marker. RESULTS: Each insult resulted in a transient and comparable decrease in food intake and fecal output consistent with the clinical picture of ileus. The endpoints were highly sensitive to small changes in low doses of endotoxin, the extent of bowel manipulation, and cerulein dose. The delay in food intake directly correlated with delayed gastric emptying. Changes in general activity and feeding behavior were insufficient to explain decreased food intake. Intestinal transit remained unchanged at the times measured. CONCLUSION: Food intake and fecal output are sensitive markers of gastric dysfunction in four experimental models of ileus. In the mouse, delayed gastric emptying appears to be the major cause of the anorexic effect associated with ileus. Gastric dysfunction is more important than small bowel dysfunction in this model. Recovery of stomach function appears to be simultaneous to colonic recovery

Expression and Cellular Immunogenicity of a Transgenic Antigen Driven by Endogenous Poxviral Early Promoters at Their Authentic Loci in MVA

CD8+ T cell responses to vaccinia virus are directed almost exclusively against early gene products. The attenuated strain modified vaccinia virus Ankara (MVA) is under evaluation in clinical trials of new vaccines designed to elicit cellular immune responses against pathogens including Plasmodium spp., M. tuberculosis and HIV-1. All of these recombinant MVAs (rMVA) utilize the well-established method of linking the gene of interest to a cloned poxviral promoter prior to insertion into the viral genome at a suitable locus by homologous recombination in infected cells. Using BAC recombineering, we show that potent early promoters that drive expression of non-functional or non-essential MVA open reading frames (ORFs) can be harnessed for immunogenic expression of recombinant antigen. Precise replacement of the MVA orthologs of C11R, F11L, A44L and B8R with a model antigen positioned to use the same translation initiation codon allowed early transgene expression similar to or slightly greater than that achieved by the commonly-used p7.5 or short synthetic promoters. The frequency of antigen-specific CD8+ T cells induced in mice by single shot or adenovirus-prime, rMVA-boost vaccination were similarly equal or marginally enhanced using endogenous promoters at their authentic genomic loci compared to the traditional constructs. The enhancement in immunogenicity observed using the C11R or F11L promoters compared with p7.5 was similar to that obtained with the mH5 promoter compared with p7.5. Furthermore, the growth rates of the viruses were unimpaired and the insertions were genetically stable. Insertion of a transgenic ORF in place of a viral ORF by BAC recombineering can thus provide not only a potent promoter, but also, concomitantly, a suitable insertion site, potentially facilitating development of MVA vaccines expressing multiple recombinant antigens